Characterization of a digestase preparation and its use to obtain primary cell cultures

Autor: Gudoshnikov, V., Baranova, I., Mamaeva, T., Fedotov, V., Balaevskaya, T., Solov'eva, N.
Zdroj: Bulletin of Experimental Biology and Medicine; June 1994, Vol. 117 Issue: 6 p593-596, 4p
Abstrakt: Abstract: The action of the preparation Digestase on several protein and synthetic substrates is examined, as well as the effect of a number of proteinase inhibitors on this enzyme preparation. Digestase has been found to exhibit considerable trypsinlike and collagenolytic activity. Experience with the use of Digestase to obtain isolated adenohypophyseal, hepatic, and cardiac cells is described, and some parameters of the functional activity of these cells in the course of their cultivation are evaluated.
Databáze: Supplemental Index