Assessment of the protective action of diphosphonate compounds against damage to T-lymphocytes by antilymphocytic serum

Autor: Zernov, I. N., Stefani, D. V., Vel'tishchev, Yu. E.
Zdroj: Bulletin of Experimental Biology and Medicine; March 1979, Vol. 87 Issue: 3 p253-254, 2p
Abstrakt: Rabbit antilymphocytic serum (ALS) and complement were taken in doses causing death of 50% of lymphocytes (stained with 1% trypan blue solution). The number of rosette-forming and transformed cells in the rosette-formation and blast-transformation tests was 60 and 24% of the number in the control respectively. After addition of 0.1 ml of 10 mM solution of the potassium salt of hydroxyethylenediphosphonic acid, belonging to the group of synthetic diphosphonates, a protective effect was observed: The number of rosette-forming and transformed lymphocytes did not differ from the control, in which the test was carried out with intact cells.
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