Immunohistochemical study of cardiolipin and phosphatidylinositol in mouse liver

Autor: Yakimenko, E. F., Beloshapkina, T. D., Khramkova, N. I., Gleiberman, A. S.
Zdroj: Bulletin of Experimental Biology and Medicine; September 1978, Vol. 86 Issue: 3 p1206-1209, 4p
Abstrakt: The localization of phospholipid haptens (cardiolipin and phosphatidylinositol) in frozen and paraffin sections of mouse liver fixed in acetone and in an acetone-buffer-formalin mixture was studied by the indirect fluorescent antibodies method. Antiphospholipid sera specifically stained the plasma membranes of the hepatocytes, especially the region of the membrane facing the blood sinus. Detection of phospholipid haptens in liver sections with the aid of antiphospholipid sera depends on the method of obtaining and fixing the sections. Depending on the method of immunization, two types of antiphospholipid sera are obtained; they differ in their stability, in the possibility of isolating antibodies on lipid immunosorbents from them, and in their ability to stain liver sections.
Databáze: Supplemental Index