| Autor: |
Davidenkova, E. F., Rozenberg, O. A., Shvarts, E. I. |
| Zdroj: |
Bulletin of Experimental Biology and Medicine; June 1977, Vol. 83 Issue: 6 p797-800, 4p |
| Abstrakt: |
To study the conditions for preparing liposomes with a high internal protein content the uptake of creatine phosphokinase by the liposomes was studied quantitatively under different experimental conditions. Addition of 20% of a positively charged lipid (stearylamine) to the lipid mixture followed by ultrasonic treatment of the liposomes for 1 min gave the highest levels of latent activity of the enzyme. Maximal uptake (6.1%) was obtained by the use of a 300 mM glucose solution, pH 7.0, containing 0.5 mg protein/ml, as the aqueous phase. Washing the preparations of liposomes with physiological saline at 205,000g gave minimal external enzyme activity. When the liposomes were kept for 7 days under argon protection at 4°C there was no decrease in the internal protein content and no increase in external activity. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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