Binding of 2-acetylaminofluorene-9-14C with nucleic acids of rat liver tissue during hepatocarcinogenesis and in primary hepatomas

Autor: Ivanov, S. D., Dzhioev, F. K.
Zdroj: Bulletin of Experimental Biology and Medicine; March 1977, Vol. 83 Issue: 3 p380-382, 3p
Abstrakt: In the course of hepatocarcinogenesis induced by 2-acetylaminofluorene (2-AAF) residual binding of 2-AAF-9-14C with rat liver nucleic acids was studied 2 weeks after a single administration of the labeled compound. Until the sixth month of administration of unlabeled 2-AAF with the diet, rat liver RNA bound an increased amount of the radioactive carcinogen, but in primary hepatomas induced by 2-AAF increased binding of the label with DNA was observed by comparison with the corresponding polynucleotides of normal liver. Prolonged binding of the carcinogen with total RNA of primary hepatomas was due mainly to its heterodisperse components with a maximum in the 18S RNA region, and also to fractions of the biopolymer with sedimentation constants of 10S and 5S, which have a high content of polyadenylate fragments.
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