Comparison of immunofluorescence and immunoperoxidase techniques for detection of tissue antigen

Autor: Asghar, Syed Shafi, Joost, Th., Cormane, R. H.
Zdroj: Archives of Dermatological Research. Archiv für Dermatologische Forschung; June 1973, Vol. 248 Issue: 2 p99-108, 10p
Abstrakt: A comparison of immunofluorescence and immunoperoxidase techniques as regards to their sensitivities to demonstrates the patterns of IgG located in the skin sections of discoid lupus erythematosus was made. Biopsies with loosely as well as densely packed IgG deposits at the dermal-epidermal junction were selected for these studies. Using the immunoperoxidase method, densely packed IgG deposits in the skin lesions could easily be detected but the technique was found to be insensitive in case IgG deposits were loosely arranged. The direct immunofluorescence technique appeared to be superior to the direct immunoperoxidase technique. The possible reason for the differences in sensitivities of the two methods is discussed.
Databáze: Supplemental Index