Observations on the appearance of neuromuscular relationships in cultured mouse tissues

Autor: Bornstein, M. B., Iwanami, H., Lehrer, G. M., Breitbart, L.
Zdroj: Cell and Tissue Research; June 1968, Vol. 92 Issue: 2 p197-206, 10p
Abstrakt: Somite preparations obtained from 10 to 16 day-old mouse embryos contained spinal cord, dorsal root ganglia and paravertebral myoblasts. These were explanted into Maximow slide assemblies and maintained for months in culture. Neurons with myelinating axons and contracting striated muscle fibers developed. In the living state, axons were observed to penetrate into areas containing mucle fibers where they appeared to terminate. Silver impregnations, histochemical demonstrations of cholinesterase activity and electron microscopic examinations of thin sections verified the development and differentiation of neuro-muscular synapses in the cultured tissue.
Databáze: Supplemental Index