| Autor: |
Keller, Svea K., Jentsch, Thomas J., Janicke, Ilse, Wiederholt, Michael |
| Zdroj: |
Pflügers Archiv European Journal of Physiology; January 1988, Vol. 411 Issue: 1 p47-52, 6p |
| Abstrakt: |
Regulation of intracellular pH (pHi) in bovine retinal pigment epithelium (RPE) was investigated in cell culture. pHi was measured using the pH-sensitive absorbance of intracellularly trapped 5 (and 6)-carboxy-dimethyl-fluorescein (CDMF). (1) Regulation of pHi after induction of an acid load by removal of NH4Cl could be blocked either totally by removal of extracellular sodium, or subtotally (about 90%) by application of amiloride (1 mmol/l). Additional flux measurements revealed a dose-dependent, amiloride-sensitive22Na+-uptake into Na+-loaded cells. Both results suggest the presence of a Na+/H+ antiport. |
| Databáze: |
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