| Abstrakt: |
Aneuploids have been produced for the 5S RNA genes of Drosophila melanogaster by three different methods. Tandem duplications were produced at a frequency of about 0.05% by irradiation of oocytes and selection for “anti-Minutes.” Other Minute suppressors were recovered and localized to the region between nuDand Pu2on the genetic map. One, SuM1, reduced crossing-over as a heterozygote and is tentatively identified as a duplication. Deficiencies for the 5S RNA genes were also produced, which showed no dominant phenotype. A Minute deficiency was localized to region 56F-57A on the salivary map and was shown to be allelic to M(2) 173. The “anti-Minute” method of selection should be useful for producing tandem duplications throughout the genome. |
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