Dependence of transfection efficiency of calcium treated Escherichia coli cells on bacterial genotype and form of lambda DNA

Autor:	Drabkina, L. E., Konevega, L. V., Legina, O. K., Mosevitsky, M. I.
Zdroj:	Molecular and General Genetics MGG; January 1976, Vol. 144 Issue: 1 p83-86, 4p
Abstrakt:	The transfecting activity of linear ? DNA is 100 times higher in calcium treated E. coli K 12 (?i434) than in non-lysogenic strains: the levels of transfection are 1–2.107 and 1–2.105 infective centers per 1 µg of ? DNA, respectively. The high efficiency of lysogenic cells transfection is not due to the spontaneously liberated “helper” phage. Evidently, it is called forth by transfecting DNA-prophage recombination or/and by inhibition of nuclease activity in lysogenic cells. Both ring forms ? DNA (supercoiled and open circles) show very low infectivity, if any, in calcinated cells.
Databáze:	Supplemental Index
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