| Autor: |
Manis, Jack J., Kline, Bruce C. |
| Zdroj: |
Molecular and General Genetics MGG; January 1977, Vol. 152 Issue: 2 p175-182, 8p |
| Abstrakt: |
The plasmids pSC138 and pML31 each contain the EcoRI-generated f5 replicator fragment of the conjugative plasmid F in addition to an EcoRI fragment encoding antibiotic resistance: ampicillin resistance derived from Staphylococcus aureus in pSC138 and kanamycin resistance from Escherichia coli in pML31. We have mapped one HindIII and two BamHI restriction sites in the f5 region of these plasmids and one HindIII site in the antibiotic resistance region of each plasmid. The HindIII site in the Km region of pML31 occurs in the kan gene whereas the HindIII site in the Ap region of pSC138 appears to occur in an area important for the regulation of ß-lactamase production. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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