| Autor: |
Orser, Cindy S., Goodner, Bradley W., Johnston, Mark, Gelvin, Stanton B., Csonka, Laszlo N. |
| Zdroj: |
Molecular and General Genetics MGG; April 1988, Vol. 212 Issue: 1 p124-128, 5p |
| Abstrakt: |
We constructed plasmids carrying the Escherichia coli proB gene that encodes ?-glutamyl kinase, under the control of the yeast GAL1 promoter. This construction was carried out with both the wild-type proB+ gene and a mutant allele, proB74, that specifies an enzyme resistant to feedback inhibition by proline. Yeast pro1 mutants harboring these plasmids are proline prototrophs. We conclude that the pro1 mutation results in a deficiency in the ?-glutamyl kinase activity in Saccharomyces cerevisiae. Expression of the proB74 allele in yeast resulted in enhanced resistance to the proline analogue l-azetidine-2-carboxylate and in a 2.4-fold elevation of the intracellular free proline levels. This result suggests that ?-glutamyl kinase is the rate limiting step in proline biosynthesis in yeast. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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