A ste12 allele having a differential effect on a versus α cells

Autor: Roche, Salome D., Shafer, Brenda K., Strathern, Jeffrey N.
Zdroj: Molecular and General Genetics MGG; January 1995, Vol. 246 Issue: 1 p80-90, 11p
Abstrakt: The transcriptional activator Ste12p is a key component of the yeast pheromone response pathway: phosphorylated as a consequence of signal transduction, it activates transcription of genes that promote mating and the subsequent fusion of the two cell types a and a. Activation by Ste12p requires three types of protein-protein interaction between DNA-binding activator proteins: (1) Ste12p by itself can induce non-cell-type-specific genes involved in mating; (2) cooperation of the transactivator Mcm1p with Ste12p induces a-specific genes; and (3) formation of a complex of the activator proteins Mcm1p and a1 (a transcriptional activator of a-specific genes) with Ste12p is believed to induce a-specific genes. We isolated and characterized a partially functional ste12 allele (ste12-T50), that is defective only in the activation of a-specific genes. ste12-T50 was isolated as a second-site mutation conferring the a mating phenotype on mata2 mutant cells. In mata2 cells, where due to the lack of repressor, a2, both sets of cell-type-specific genes are expressed, ste12-T50 apparently tips the balance in favor of a-specific gene expression. Thus, mata2 ste12-T50 cells mate like a cells. Additional ste12 mutants that confer the a mating phenotype on mata2 cells have also been isolated.
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