Immunoelectron microscopy of the bone marrow mononuclears labelling with rabbit anti-mouse brain serum using peroxidase-anti-peroxidase method

Autor: Butenko, Z. A., Zak, K. P., Komissarenko, S. V., Gruzov, M. A., Khomenko, B. M.
Zdroj: Annals of Hematology; December 1983, Vol. 47 Issue: 6 p343-349, 7p
Abstrakt: Ultrastructural features of mouse bone marrow mononuclears (BMM) labelled with peroxidase-anti-peroxidase (PAP) complex using rabbit anti-mouse brain serum (RAMBS) and rabbit anti-mouse brain serum, absorbed with thymocytes (RAMBST) were investigated. After incubation of cells with RAMBST which have high activity against mouse pluripotent hemopoietic stem cells (CFUs) about 0.6% of BMM were labelled. According to their fine structure these cells were similar to those described earlier as presumptive pluripotent hematopoietic stem cells (PHSC). After incubation with RAMBS 1.9% of BMM were labelled, including cells marked by RAMBST and typical small lymphocytes with ultrastructure of T-lymphocytes.
Databáze: Supplemental Index