Detection of lectins in nodulated peanut and soybean plants

Autor: Kishinevsky, B. D., Law, I. J., Strijdom, B. W.
Zdroj: Planta; November 1988, Vol. 176 Issue: 1 p10-18, 9p
Abstrakt: The direct double-antibody enzymelinked immunosorbent assay system was used in the detection and measurement of seed lectins from peanut (Arachis hypogaea L.) and soybean (Glycine max L.) plants (PSL and SBL, respectively) that had been inoculated with their respective rhizobia. Concentrations of PSL dropped to undetectable levels in peanut roots at 9 d and stems and leaves at 27 d after planting; SBL could no longer be detected in soybean roots at 9 d and in stems and leaves at 12 d. A lectin antigenically similar to PSL was first detected in root nodules of peanuts at 21 d reaching a maximum of 8 µg/g at 29 d then decreasing to 2.5 µg/g at 60 d. There was no evidence of a corresponding lectin in soybean nodules.
Databáze: Supplemental Index