| Autor: |
Kojima, Naoshi, Takebayashi, Toshie, Mikami, Akiko, Sugino, Maiko, Ohtsuka, Eiko, Komatsu, Yasuo |
| Zdroj: |
Nucleic Acids Symposium Series; September 2008, Vol. 52 Issue: 1 p463-464, 2p |
| Abstrakt: |
We previously reported a series of new amino-linkers, consisting of an aminoethyl carbamate structure (Komatsu, 2008). We have now examined the chemical properties of oligonucleotides modified with an ssH-linker, which is the simplest and most cost-effective derivative of the series. Although it was previously shown that monomethoxytrityl protection on a primary amine of the ssH-linker was cleaved under weakly acidic conditions (1% acetic acid), we found that the deprotection also proceeded in aqueous buffer solutions (pH 6.0, 7.0). The MMT group was removed much faster than other commercially available amino-linkers, and this property enabled the ssH-modified oligonucleotides to be conveniently purified with a cartridge column. Furthermore, the ssH-modified oligonucleotides were utilized in on-support labeling reactions. As compared with other amino-linkers, the ssH-linker was superior in terms of its purification and reaction efficiencies. |
| Databáze: |
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