| Abstrakt: |
A variant of the use of reversed-phase high-performance liquid chromatography is described which permits the separation of pantothenic acid derivatives. The stationary phase used was a μBondapak-C18(4.1 × 250 mm column; 4.6 × 50 nm precolumn). Elution was performed in the isocratic regime using as mobile phase 20 M potassium phosphate buffer (pH 5.0)-methanol (91.5:8.5). The rate of elution was 1 ml/min. Retention times in the column for phosphopantothenate, pantothenate, phosphopantetheine, CoA, and dephosphoCoA were about 3.5, 6, 10.5, 16, and 42 min, respectively. This method, with radioactive detection, can be used for the analysis of pantothenic acid derivatives in liver extracts. One hour after white rats had been injected with [14C]pantothenic acid, the abovementioned components (with the exception of dephosphoCoA) contained the label in a ratio of 4:18:54:24. |
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