Synthesis of uridine diphosphate [1-3H]glucose and uridine diphosphate [6-3H]glucose

Autor: Snetkova, E. V., Akulov, G. P., Gordeeva, L. S., Kaminskii, Yu. L.
Zdroj: Chemistry of Natural Compounds; January 1987, Vol. 23 Issue: 1 p105-108, 4p
Abstrakt: Information is given on the synthesis of tritium-labeled UDPG by the successive transformation of D-[1-3H]glucose or D-[6-3H]glucose by the action of the enzymes hexokinase (EC 2.7.1.1), phosphoglucomutase (EC 2.7.5.1), and UDPG-pyrophosphorylase (EC 2.7.7.9) under conditions ensuring the retention of the tritium label. Methods of obtaining tritium-labelled substrates and intermediate products of their enzymatic transformations in the synthesis of [3H]UDPG are discussed.
Databáze: Supplemental Index