Immunogold localization of a developmentally regulated, tapetal-specific, 15 kDa lily anther protein

Autor: Balsamo, R. A., Wang, J. -L., Eckard, K. J., Wang, C. -S., Lord, E. M.
Zdroj: Protoplasma; March 1995, Vol. 189 Issue: 1-2 p17-25, 9p
Abstrakt: Summary We have confirmed that the LLA-15 polypeptide ofLilium longiflorum is (a) tapetum specific with some expression possible in the adjacent middle layer cells and (b) relatively abundant as evidenced by the high density of gold particles localized to the tapetal cells. We have established that the protein is cytoplasmic and not associated with organelles, membranes, extracellular matrix or wall. We also report an amino acid composition of the molecule and a partial sequence which bears no resemblance to any protein yet described.
Databáze: Supplemental Index