| Autor: |
Large, A. T., Jones, Carolyn J. P., Connock, M. J. |
| Zdroj: |
Protoplasma; September 1993, Vol. 175 Issue: 3-4 p93-101, 9p |
| Abstrakt: |
Summary Post nuclear supernatants prepared from homogenates of the digestive gland of the slugArion ater were analysed by rate-dependent and density-dependent density gradient zonal centrifugation. Mannitol oxidase carrying structures exhibited distinct centrifugal behaviour sedimenting more slowly than mitochondria, lysosomes and peroxisomes but faster than microsomes, and banding sharply at a density of 1.15 g/ml in sucrose gradients. By combining rate and isopycnic centrifugations mannitol oxidase carrying structures were largely separated from contaminating organelles. Electron microscopic examination of such mannitol oxidase-enriched fractions revealed the predominant presence of distinct structures of tubular form. SDS PAGE analysis indicated that the major polypeptide present had a mass of ~ 68 kDa corresponding to the major subunit previously reported for partially purified mannitol oxidase ofHelix aspersa. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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