Electron microscopic visualization of membrane Ca2+-binding sites in barley root tip cells: Inductionin vivo by seed germination in the presence of CaCl2

Autor:	Belitser, N. V., Zaalishvili, G. V.
Zdroj:	Protoplasma; June 1983, Vol. 115 Issue: 2-3 p222-227, 6p
Abstrakt:	Summary Germination of barley seeds in the presence of 1 mM, 10 mM or 50 mM CaCl2 induces formation of electron-dense membrane-associated globules, whose structure and distribution closely resemble those observed using the cytochemical Oschman-Wall's technique. Treatment of thin sections by pronase and phospholipase A solutions lead to a partial dissolution of intramembranous dense material. The images obtained are similar to those of samples, germinated in distilled water but subjected to calcium-supplemented fixation and washing procedures during tissue processing for electron microscopy.
Databáze:	Supplemental Index
Externí odkaz:	Zobrazit plný text záznamu Full text from SpringerLink