| Autor: |
Kalnitsky, G., Singh, H., Kuo, T., Ihnen, J., Clarke, W. R., Ratajczak, H. V., Richerson, H. B. |
| Zdroj: |
Lung; December 1982, Vol. 160 Issue: 1 p245-256, 12p |
| Abstrakt: |
Proteases are among the potentially toxic agents that may be produced by lung cells during nonspecific or specific immunologic inflammation. We studied levels of selected intracellular lysosomal proteases in an established rabbit model of acute hypersensitivity pneumonitis to determine if demonstrable changes in these proteases occurred. Cathepsins B1, B2, C and D, dipeptidylaminopeptidase II and elastolytic enzyme in lung homogenates and subcellular fractions were quantitated using specific substrates, and reported as total activity (per lung) and as specific activity (activity per mg protein). Rabbits undergoing acute inflammation had significantly increased total activity for cathepsins B1, B2, C and D in lysosomal fractions compared to controls, with increased total activity of cathepsins B1, C and D in cytoplasmic fractions as well. Specific activity was significantly increased for cathepsins B1, B2 and C in the lysosomal compartment and of cathepsin C in the cytoplasmic fraction. The intracellular lung proteases exhibited a variety of changes during systemic immunization, inhalation of aerosolized antigen and recovery from inflammation. The cellular origin of these enzymes and whether their changes contribute to injury, aid in recovery, or are simply epiphenomena remain to be determined. |
| Databáze: |
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