| Autor: |
White, D. C., Davis, W. M., Nickels, J. S., King, J. D., Bobbie, R. J. |
| Zdroj: |
Oecologia; January 1979, Vol. 40 Issue: 1 p51-62, 12p |
| Abstrakt: |
The measurement of lipid phosphate is proposed as an indicator of microbial biomass in marine and estuarine sediments. This relatively simple assay can be performed on fresh, frozen or frozen-lyophilized sediment samples with chloroform methanol extraction and subsequent phosphate determination. The sedimentary lipid phosphate recovery correlates with the extractible ATP and the rate of DNA synthesis. Pulse-chase experiments show active metabolism of the sedimentary phospholipids. The recovery of added 14C-labeled bacterial lipids from sediments is quantitative. Replicate analyses from a single sediment sample gave a standard deviation of 11%. The lipid extract can be fractionated by relatively simple procedures and the plasmalogen, diacyl phospholipid, phosphonolipid and non-hydrolyzable phospholipid content determined. The relative fatty acid composition can be readily determined by gas-liquid chromatography. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full text from SpringerLink