| Autor: |
Kerby, Nigel W., Musgrave, Stephen C., Rowell, Peter, Shestakov, Sergey V., Stewart, William D. P. |
| Zdroj: |
Applied Microbiology and Biotechnology; April 1986, Vol. 24 Issue: 1 p42-46, 5p |
| Abstrakt: |
Ethylenediamine (EDA) is toxic to the cyanobacterium Anabaena variabilis and inhibits nitrogenase activity. The inhibition of nitrogenase was prevented by pretreatment of cells with l-methionine-d,l-sulphoximine (MSX). Mutant strains of Anabaena variabilis (ED81, ED92), resistant to EDA, had low levels of glutamine synthetase (GS) biosynthetic activity compared with the wild type strain. ED92 had a low level of GS protein whereas ED81 had a similar level to that of the parent strain as estimated using antibodies against GS. Both strains fixed N2 and liberated NH4+ into the media. Following immobilization of the mutant strains, sustained photoproduction of NH4+ was obtained in air-lift reactors at rates of up to 50 µmol NH4+ mg chl a-1 h-1, which were comparable to the rates obtained when immobilized cyanobacteria were treated with MSX. |
| Databáze: |
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