Characterization of cytoplasmic male sterility in Petunia hybrida. I. Localization, composition and activity of esterases

Autor: Marrewijk, G. A. M., Bino, R. J., Suurs, L. C. J. M.
Zdroj: Euphytica; March 1986, Vol. 35 Issue: 1 p77-88, 12p
Abstrakt: Anthers of male fertile, cytosterile and restored male fertile clones of Petunia hybrida were compared for esterase activity and composition in subsequent stages of microsporogenesis. Three methods were applied (i) ultra-thin layer isoelectric focussing on polyacryl amide gels, (ii) quantitative spectrophotometrical assay, (iii) histochemical determination of total esterase activity associated to the azo-coupling method (Pearse, 1972).
Databáze: Supplemental Index