VLA-4-dependent adhesion activities of U937 cells and guinea pig bronchoalveolar lavage leukocytes

Autor: Monshizadegan, D. A., Holloway, D. A., Torrente, J. M., Yednock, T., Fritz, L., Sturm, R. J.
Zdroj: Inflammation Research; March 1993, Vol. 39 Issue: Supplement 1 pC177-C179, 3p
Abstrakt: VLA-4-dependent binding to fibronectin (FN) and to a human vascular cell adhesion molecule (hVCAM-1)-transfected murine cell line was measured using U937 cells and guinea pig (GP) bronchoalveolar lavage (BAL) cells. A species cross-reactive, blocking monoclonal antibody directed against human VLA-4 (TY 21.6) inhibited U937/FN binding by 71±7%. The presence of TY 21.6 inhibited the stimulated binding of U937 cells to hVCAM-1 by 84%. However, TY 21.6 was unable to inhibit the BAL/FN binding. With the addition of TY 21.6, the binding of PMA-stimulated BAL cells to hVCAM-1 was inhibited by 57±5%. In summary, human and guinea-pig leukocytes express binding activity to both FN and hVCAM-1. A specific VLA-4 blocking monoclonal antibody, TY 21.6, inhibited U937 and BAL cell binding to hVCAM-1, but only inhibited FN binding with U937 cells.
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