| Autor: |
Werf, M. J., Hartmans, S., Tweel, W. J. J. |
| Zdroj: |
Applied Microbiology and Biotechnology; 19950801, Vol. 43 Issue: 4 p590-594, 5p |
| Abstrakt: |
Pseudomonas pseudoalcaligenes can only form d-malate from maleate after incubation of the cells with a solvent or a detergent. The effect of the detergent Triton X-100 on d-malate production was studied in more detail. The longer the cells were incubated with Triton X-100, the higher was the d-malate production activity, until the maximal malease activity was reached. Incubation of P. pseudoalcaligenes cells with Triton X-100 also resulted in an increase in the protein concentration of the supernatant, indicating that cell lysis had occurred. The rate at which the d-malate production activity increased was dependent on the Triton X-100 concentration and on the cell density. Also the rate at which lysis occurred depended on the Triton X-100 concentration. |
| Databáze: |
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