| Autor: |
Gray, Murray R., Banerjee, Dwijen K., Fedorak, Phillip M., Hashimoto, Atsumi, Masliyah, Jacob H., Pickard, Michael A. |
| Zdroj: |
Applied Microbiology and Biotechnology; 19940201, Vol. 40 Issue: 6 p933-940, 8p |
| Abstrakt: |
Soil impregnated with anthracene was subjected to bacterial treatment as a model for remediation of soils contaminated with polycyclic aromatic hydrocarbons (PAHs). Roller bottles were used to simulate the mixing of solids in rotating drums. The soil was prepared as a 60 weight % slurry in salts medium, and inoculated with a mixed culture, selected for the ability to mineralize anthracene as its sole carbon source. The degradation of anthracene followed zero-order kinetics when it was well dispersed on the soil particles, consistent with a rate limitation by dissolution of solid hydrocarbon. Maximum degradation rates of 300 µg/g per day were achieved in repeated batch operation, using a 10% volume of soil slurry as the inoculum for the succeeding batch. Anthracene degradation activity was maintained through 18 such transfers on a 3- to 4-day cycle. The culture produced soluble compounds that enhanced the solubility of anthracene in aqueous solution. Soil components did not have a significant effect on growth or degradation. Degradation of anthracene ceased when the concentration in soil dropped to approx. 30 µg/g, from an initial value of 600 µg/g. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full text from SpringerLink