| Autor: |
Mirhabibollahi, Bahram, Brooks, Joy L., Kroll, Rohan G. |
| Zdroj: |
Applied Microbiology and Biotechnology; November 1990, Vol. 34 Issue: 2 p242-247, 6p |
| Abstrakt: |
A semi-homogeneous amperometric immunosensor specific to the protein A of Staphylococcus aureus was developed using direct electrochemical detection of phenol produced by alkaline phosphatase from phenyl phosphate. The immunosensor could reliably detect strains of protein A-bearing S. aureus in pure cultures at ca. 304 cfu/ml, and at ca. 105 cfu/g or ml in various food samples. Due to its semi-homogeneous nature, the system was very simple, easy to operate, and labour-saving. The good correlation between the amperomatric current generated by the immunosensor and plate counts illustrated the potential usefulness of this simple system. It proved to be a reliable 24-h detection method for food samples containing very low numbers of protein A-bearing S. aureus after pre-enrichment, as it was able to detect cells that could not directly be enumerated by plate counts. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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