Excessive production of ammonium from nitrate by some methanol-assimilating yeast strains

Autor: Middelhoven, W. J., Berends, J., Repelius, C., Aert, A. J. M.
Zdroj: Applied Microbiology and Biotechnology; September 1976, Vol. 2 Issue: 3 p169-173, 5p
Abstrakt: Some strains ofCandida boidinii excrete ammonium when grown in nitrate methanol medium under conditions of oxygen limitation but not in wellaerated cultures or under anaerobic conditions. With other carbon sources ammonium excretion is observed only in the late exponential and in the stationary growth phase. Ammonium excretion by methanol-grown cultures approximately equals assimilatory nitrate reduction. The share of total nitrate reduction in the electron transport of methanol-grown cultures is about 18%. Increase in cell yield of oxygen-limited cultures due to nitrate addition was not observed. Nitrate reduction byC. boidinii appears to be not of the true dissimilatory type. Nor is it restricted to the assimilatory type: it is not inhibited or repressed by ammonium and the ammonium excretion occurs in quantities too large to be attributed to amino acid degradation.
Databáze: Supplemental Index