Observations of a naturally-occurring lattice array of ribosomes in ultrathin section of ajellomyces dermatitidis

Autor: Garrison, Robert G., Boyd, Karen S.
Zdroj: Mycopathologia; November 1974, Vol. 54 Issue: 3 p335-339, 5p
Abstrakt: A highly organized crystalline lattice array believed to represent an intact naturally-occurring polyrisobome was observed in ultrathin section of a hyphal cell ofAjellomyces dermatitidis. This remarkable structure was composed of approximately 120 ribosomal particles in a high degree of 3-dimensional order. Connecting fibers thought to be informational ribonucleic acid were clearly demonstrated between the adjacent ribosomal particles of the lattice array. Subsequent attempts to induce ordered arrays of ribosomes in the hyphae ofA. dermatitidis by pre-cooling and treatment with physiological concentrations of vinblastine sulfate were not successful. It was concluded that the demonstration of naturally-occurring polyribosomes in this fungal organism by conventional techniques of electron microscopy was entirely fortuitous, and that such observation in ultrathin sections is an exceedingly rare event. Further, we believe this report to be the first demonstration by electron microscopy of an intact naturally-occurring polyribosome from fungal tissue.
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