Localization of the 5S RNA genes in Zea mays by RNA-DNA hybridization in situ

Autor: Wimber, D. E., Duffey, Patricia A., Steffensen, D. M., Prensky, W.
Zdroj: Chromosoma; December 1974, Vol. 47 Issue: 4 p353-359, 7p
Abstrakt: 5S RNA was extracted from Zea mays tissue and iodinated in vitro with 125I to a high specific activity. Acrylamide gel electrophoresis of the 125I-5S RNA, 11/2 weeks after iodination demonstrated that most of the 5S RNA molecules were degraded to half-size or smaller. In situ hybridization with this iodinated RNA to pachytene microsporocyte chromosomes showed that the 5S RNA cistrons are located near the end of the long arm of chromosome 2. No obvious association of the 5S locus with the nucleolus was seen during pachytene or later stages.
Databáze: Supplemental Index