| Autor: |
Chistoserdova, L. V., Chistoserdov, A. Y., Schklyar, N. L., Baev, M. V., Tsygankov, Y. D. |
| Zdroj: |
Antonie van Leeuwenhoek; August 1991, Vol. 60 Issue: 2 p101-107, 7p |
| Abstrakt: |
In methanol-limited continuous cultures of the obligate methylotrophic bacteriumMethylobacillus flagellatum grown at rates from 0.05 to 0.63 h-1, and also in an oxyturbidostat culture ofM. flagellatum growing at the rate of 0.73 h-1, levels of methanol dehydrogenase, enzymes of formaldehyde oxidation (both linear and cyclic) and assimilation (RuMP cycle), a number of intermediary metabolism and TCA cycle enzymes and also ‘dye-linked’ formaldehyde dehydrogenase were determined. It was shown that the activities of dissimilatory enzymes, with the exception of ‘dye-linked’ formaldehyde dehydrogenase, decreased with increasing growth rate. Activities of assimilative enzymes and activities of the TCA cycle enzymes detected as well as the ‘dye-linked’ formaldehyde dehydrogenase activity, increased with increasing growth rate. A periplasmic location was shown for the latter enzyme and a role in formaldehyde detoxification was proposed. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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