Autor: |
Veenhuis, M., Hoogkamer-Te Niet, M. C., Middelhoven, W. J. |
Zdroj: |
Antonie van Leeuwenhoek; January 1985, Vol. 51 Issue: 1 p33-43, 11p |
Abstrakt: |
Growth of Candida famata and Trichosporon cutaneum on uric acid as the sole source of carbon and nitrogen was associated with the development of a number of microbodies in the cells. Cytochemical staining experiments showed that the organelles contained urate oxidase, a key enzyme of uric acid metabolism, and catalase. Transfer of cells, precultured on glucose or glycerol, into uric acid-containing media indicated that these microbodies originated from the organelles, originally present in the inoculum cells, by growth and division. In urate-grown C. famata the microbodies were frequently observed in large clusters; in both organisms they existed in close association with mitochondria and strands of ER. The organelles lacked crystalline inclusions. In freeze-fractured cells their surrounding membranes showed smooth fracture faces. |
Databáze: |
Supplemental Index |
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