| Autor: |
Golino, M. D., Hamill, O. P. |
| Zdroj: |
Journal of Membrane Biology; September 1992, Vol. 129 Issue: 3 p297-309, 13p |
| Abstrakt: |
This study examines the subunit requirement for Torpedo acetylcholine receptor (AChR) channel expression and the influence of non-a-subunit deletions on single AChR-channel currents. Xenopus oocytes injected with subunit combinations deficient in single non-a-subunit mRNA transcripts display the following order of ACh sensitivity: ß-less > ?-less > d-less. Oocytes injected with only the a-subunit and one non-a-subunit display the order: ad > a? > aß. These sequences indicate the effectiveness of non-a-subunit substitution is d > ? > ß. Single AChR-channel currents measured in oocytes deficient in either ß or ? display conductance and voltage-sensitive burst kinetics similar to the wild-type channel. In contrast, the d-less combination express channels with burst kinetics that are relatively faster and voltage insensitive. These results indicate that either a specific structural domain in the d-subunit or its specific interactions with the a-subunit contribute to the voltage-dependent gating of the Torpedo AChR channel. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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