| Autor: |
Beeler, Troy J., Dux, Laszlo, Martonosi, Anthony N. |
| Zdroj: |
Journal of Membrane Biology; February 1984, Vol. 78 Issue: 1 p73-79, 7p |
| Abstrakt: |
Summary Two-dimensional crystalline arrays of Ca2+-ATPase molecules develop after treatment of sarcoplasmic reticulum vesicles with Na3VO4 in a Ca2+-free medium. The influence of membrane potential upon the rate of crystallization was studied by ion substitution using oxonol VI and 3,3'-diethyl-2,2'-thiadicarbocyanine (Di-S-C2(5)) to monitor inside positive or inside negative membrane, potentials, respectively. Positive transmembrane potential accelerates the rate of crystallization of Ca2+-ATPase, while negative potential disrupts preformed Ca2+-ATPase crystals, suggesting an influence of transmembrane potential upon the conformation of Ca2+-ATPase. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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