PCR-generated cDNA library of transition-stage maize embryos: cloning and expression of calmodulin genes during early embryogenesis

Autor: Breton, Christian, Chaboud, Annie, Matthys-Rochon, Elisabeth, Bates, Elizabeth E. M., Cock, J. Mark, Fromm, Hillel, Dumas, Christian
Zdroj: Plant Molecular Biology; January 1995, Vol. 27 Issue: 1 p105-113, 9p
Abstrakt: One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3 × 105 recombinants and two different calmodulin cDNAs were cloned using a heterologous probe from petunia. Calmodulin expression was confirmed throughout maize embryogenesis at the mRNA, amplified cDNA and protein levels. Sequence analysis suggests a maize origin for both clones and negligible nucleotide changes linked to PCR. This library is the first described for early plant embryos and represents a breakthrough to isolate genes involved in embryo differentiation.
Databáze: Supplemental Index