| Autor: |
Strand, Roger, Bøe, Roald, Flatmark, Torgeir |
| Zdroj: |
Molecular and Cellular Biochemistry; October 1994, Vol. 139 Issue: 2 p149-157, 9p |
| Abstrakt: |
Standard protocols forin vitro transcription assay (nuclear run-off) include 10–40% (v/v) glycerol (of various ionic strength) in the medium used for resuspension/storage of the isolated nuclei. In the present work the morphological and functional properties of nuclei isolated from rat liver have been studied as a function of the content of glycerol, sucrose and inorganic ions (K+ and Mg2+) in the resuspension medium. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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