Autor: |
Nikolskaya, Irina I., Aleksandrova, Svetlana S., Lopatina, Nadezgda G., Debov, Sergej S. |
Zdroj: |
Molecular and Cellular Biochemistry; June 1978, Vol. 20 Issue: 1 p17-24, 8p |
Abstrakt: |
Specificity of DNA methylation enzymes from the E. coli SK cells and conditions for their separation have been investigated. Column chromatography on carboxymethylcellulose permits fractionation of methylase activity into six discrete peaks whose specificity to the methylated base has been determined in vitro with H3-SAM as precursor. All methylases specific for adenine produced 6'-methylaminopurine, all methylases specific for cytosine yielded 5'-methylcytosine. |
Databáze: |
Supplemental Index |
Externí odkaz: |
|