Subcellular localization of glucose-6-phosphate dehydrogenase in tobacco mesophyll protoplasts

Autor: Šlndelářová, Milada, Šlndelář, L.
Zdroj: Biologia Plantarum; March 1991, Vol. 33 Issue: 2 p150-155, 6p
Abstrakt: Subcellular localization of glucose-6-phosphate dehydrogenase (EC 1.1.1.49.) isoenzymes was determined in mesophyll protoplasts prepared from Nicotiana tabacum L. cv. Samsun. Intact chloroplasts and soluble cytosolic proteins were obtained by means of differential centrifugation. The 1000 g pellet contained 97 % of chloroplasts and 16.8 ± 2.1 % of the total activity of glucose-6-phosphate dehydrogenase. The rest of the enzyme was localized in the cytosol which also contained 91 % of the total activity of phosphoenolpyruvate carboxylase.
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