Autor: |
Parthier, B., Lerbs, S., Lehmann, J., Wollgiehn, R. |
Zdroj: |
Biologia Plantarum; March 1985, Vol. 27 Issue: 2-3 p131-138, 8p |
Abstrakt: |
Treatment of etiolated and excisedCucurbitacotyledons with exogenous cytokinin (benzyladenine) in darkness or light results in a marked stimulation of Rubisco activity, content of enzyme protein, and incorporation of labelled precursors into it, indicating cytokinin-stimulatedde novosynthesis of the enzyme. Cell-free translations of RNA in the wheat germ andE. colisystems show an increase in both large and small subunit mRNA amounts relative to the increase of total RNA under the influence of the phytohormone and light. This increase in the level of translatable RNA is confirmed by RNA hybridization with the Rubisco large subunit gene of spinach. In addition, our results demonstrating additive effects of benzyladenine and light in cotyledon and chloroplast development suggest that the two factors co-act independently in the causal sequence of Rubisco gene expression. The data are discussed in a general view of cytokinin action in gene expression steps. |
Databáze: |
Supplemental Index |
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