| Autor: |
Jermoljev, Evžen, Albrechtová, Liana |
| Zdroj: |
Biologia Plantarum; November 1966, Vol. 8 Issue: 6 p434-443, 10p |
| Abstrakt: |
Abstract: The inhibitor of the X virus (Jermoljev, Albrechtová 1965) can be separated from other proteins by 60 min centrifugation at 40000 r.p.m. in the Spinco L centrifuge and by subsequent gel filtration of the supernatant on a Sephadex G-50 column. For the elution it is best to use Tris-maleate buffers and 0·2M Tris-HCl of pH 7·2. The inhibitor is present in the first fractions containing proteins, between 30 and 45 ml. It is most suitable to concentrate the inhibitor by salting out with ammonium sulphate to 30–50% saturation. The inhibitor can be preserved by adding ammonium sulphate to the sap. At 3°C it can be kept in this manner for 2 months. If the pH of the sap drops below 5·5, the inhibitor is inactivated. Complete inactivation of the inhibitor takes place after a 10 min heating at 67·5°C. No substantial difference in the amount of inhibitor in the sap from apical leaves and leaves from middle levels has been found. Keeping the plants in the dark has no effect on the inhibitor level. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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