| Autor: |
Sugiyama, Masanori, Mizuno, Seiji, Ohta, Yuichi, Mochizuki, Hiroshi, Nimi, Osamu |
| Zdroj: |
Biotechnology Letters; January 1990, Vol. 12 Issue: 1 p1-6, 6p |
| Abstrakt: |
Streptomycin (SM)-producingStreptomycesgriseuswas permeable to extracellular SM during exponential growth, and less permeable during the stationary phase when antibiotic production was maximal. Uptake of [3H] dihydrostreptomycin ([3H]DSM) by the producer organism was abolished by inhibitors of electron transport, sulfhydryl reagents and an uncoupler of oxidative phosphorylation, and it was competitively inhibited by spermidine. These results indicate that SM was taken up by an active transport process via a polyamine transport system. A mutant with lower SM-resistance showed the same level of SM 6-phosphotransferase as the parent strain. It is suggested that selfresistance in the SM-producers is at least partly determined by transport and permeability mechanisms. |
| Databáze: |
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| Externí odkaz: |
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