Immune responses in vitro

Autor:	Click, Robert E., Schneider, David, Sitzmann, Lisa A., Azar, Miguel M.
Zdroj:	Immunogenetics; September 1984, Vol. 20 Issue: 3 p301-310, 10p
Abstrakt:	The Mls locus was originally defined to have four alleles; three controlled products that were detectable in primary mixed leukocyte reactions (MLR), whereas one, b, was described as being null. Recently, other investigators postulated that the Mls locus is nonpolymorphic, being composed of the b null allele and of a singly expressed allele previously thought to be the a and d alleles. We previously reported that products controlled by Mls^aand Mls^dwere antigenically distinct and therefore are not controlled by the same allele, and the product of Mls^bon cells of three different strains was easily detectable by Mls^aand Mls^dresponding cells. Thus the b allele is not null. In the present report evidence is presented which indicates that both Mls^band Mls^cencoded products were undetectable by MLR when in the presence of Mls^aor Mls^d. This was demonstrated by (a) the inability of Mls^a/Mls^cand Mls^a/Mls^bF1 cells to stimulate Mls^aresponding cells and Mls^d/Mls^cand Mls^d/Mls^bcells to stimulate Mls^dcells; (b) the positive response of Mls^a/Mls^band Mls^d/Mls^bF1-hybrid cells to Mls^b-encoded products; and (c) the reactivity of Mls^a/Mls^cand Mls^d/Mls^cF1 hybrid cells to Mls^c-encoded determinants.
Databáze:	Supplemental Index
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