| Autor: |
Dwyer, Barney E., Donatoni, Paul, Wasterlain, Claude G. |
| Zdroj: |
Neurochemical Research; December 1982, Vol. 7 Issue: 12 p1507-1514, 8p |
| Abstrakt: |
Following the injection of 4-day old rats with 150 mMl-[3,4-3H]valine (10µmol/g, IP) the incorporation of3H into protein was linear 2 hours. Valine specific activity in the brain acid-soluble fraction was constant between 30 and 120 min after injection with a mean value of 82.3% of the injectate. Significant amounts of tritated metabolites accumulated in the brain acid-soluble fraction (41.4% of radioactivity at 120 min) but do not prove an impediment to measuring rates of protein synthesis. The rate of protein synthesis in cerebral cortex of the 4-day old rat was measured by quantitative autoradiography using [3H]valine and3H-sensitive film. The measured rate shows excellent agreement with that found previously usingl-[1-14C]valine. Our results suggest that [3H]valine can be a useful precursor to measure local rates of brain protein synthesis by quantitative autoradiography. |
| Databáze: |
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