| Autor: |
Ennulat, David J., Stach, Robert W. |
| Zdroj: |
Neurochemical Research; July 1985, Vol. 10 Issue: 7 p1009-1014, 6p |
| Abstrakt: |
An improved procedure for the isolation of iodinated ß Nerve Growth Factor (125I-ßNGF) has been devised. Use of Centricon microconcentrators (Amicon) has allowed the facile and efficient recovery of ultrapure125I-ßNGF in high yields. Centricon microconcentrators are supplied with two molecular weight cutoffs of 10 K and 30 K daltons. ßNGF is a basic protein with a molecular weight of 26 K daltons. It is therefore possible to filter the125I-ßNGF through the 30K filter (30 K Filtrate) leaving behing any aggregates or reactants greater than 30 K while the125I-ßNGF can be retained and concentrated on the 10 K filter (10 K Retentate). Any free125I is easily removed, passing through the 10 K filter and then being discarded. In this way125I-ßNGF can be easily purified. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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