| Autor: |
BARJA, FRANÇOIS, JR, ARY CORRÊA, STAPLES, R. C., HOCH, H. C. |
| Zdroj: |
Mycological Research; December 1998, Vol. 102 Issue: 12 p1513-1518, 6p |
| Abstrakt: |
Germlings of Uromyces appendiculatus respond to topographical stimuli and develop appressoria. During this period several genes are expressed, one of which is Inf24. Germlings were microinjected with a sense and antisense fragment of the ORF of Inf24. Sense (Inf24-S), 5'-ATG AGT GCT TCA TCG CAG CAG CAG-3' fragment did not affect germling response to the topographical stimuli and the developed appressoria. Microinjection of antisense (Inf24-RC), 5'-CTG CTG CTG CGA TGA AGC ACT CAT-3' blocked gene transcription and appressoria were rarely formed. Injection of Inf24-RC into already formed appressoria did not inhibit continued development of subsequent infection structures, e.g. penetration peg, sub-stomatal vesicle. |
| Databáze: |
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