Phospholipids and phospholipid-bound sodium ions in the outer segments of The retinal rods

Autor: Leont'ev, V. G., Berman, A. L., Etingof, R. N.
Zdroj: Neuroscience and Behavioral Physiology; July 1972, Vol. 5 Issue: 3 p251-258, 8p
Abstrakt: The chief phospholipids of the outer segments are phosphatidyl-ethanol-amine, lecithin, and phosphatidyl-serine. The conditions of extraction (temp-erature, solvent) have a substantial effect on the yield and qualitative composition of the extracted phospholipids. After low-temperature (-35C) extraction with a mixture of N-hexane and chloroform (1:2) 60–80% of the phospholipids of the outer segments were extracted (phosphatidyl-ethanolamine was extracted almost completely, phosphatidyl-serine about 80%, and lecithin about 60%). The Na+ concentration in these extracts was much higher than that of K+, and the Na+/K+ ratio for them was 4. 0, compared with 0. 6 for the initial fractions of the outer segments.
Databáze: Supplemental Index