Abstrakt: |
Genetic studies were performed on octanol dehydrogenase (EC 1.1.1.73) and a-glycerophosphate dehydrogenase (EC 1.1.1.8) in the mosquito Aedes (Finlaya) togoi by agar gel electrophoresis. The electrophoretic survey revealed two octanol dehydrogenase loci (Odh-1, Odh-2) and one a-glycerophosphate dehydrogenase locus (a-Gpdh) in this species. Five alleles were observed at the Odh-2 locus in seven laboratory strains, whereas the a-Gpdh locus was completely monomorphic in six of seven strains examined and the second allele at this locus was detected in only one strain at a frequency of 0.14. Both loci code for dimeric enzymes. Linkage studies on Odh-2 and a-Gpdh suggested that the gene arrangement and recombination units were Odh-2—(25.8)—M (sex locus)—(30.5)—s (strawcolored larva) and M—(25.6)—a-Gpdh—(15.4)—s. These results, together with linkage data previously reported, give the following gene linkage on the sex chromosome: Odh-2—Est-3 (carboxylesterase)—Acph (acid phosphatase)—M—a-Gpdh—s—Est-2 (carboxylesterase). The total map length of this arrangement is approximately 75 map units. |