| Autor: |
Cyr, David R., Lazaroff, Wayne R., Grimes, Susanna M. A., Quan, Grant, Bethune, Terry D., Dunstan, David I., Roberts, Dane R. |
| Zdroj: |
Plant Cell Reports; July 1994, Vol. 13 Issue: 10 p574-577, 4p |
| Abstrakt: |
Embryogenic cultures of interior spruce derived from 12 full-sib families were subjected to cryopreservation, with a 97 % success rate for 357 genotypes. Analyses suggested that cryotolerance was not related to family ranking (height increment), embryogenic potential or culture dispersability in suspension, and long-term storage in or above liquid nitrogen did not affect regenerative potential. By contrast, differences in cryotolerance among cell lines appeared to be prevalent in certain families. Analysis with a DNA fingerprinting probe used for clonal identification demonstrated no evidence of somaclonal variation as a result of cryopreservation. The results of this work indicate the applicability of cryopreservation as a long-term storage strategy for spruce embryogenic cultures from a wide genetic background. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full text from SpringerLink